Application

Research Sub CategoryNuclear Receptors

Research CategoryEpigenetics & Nuclear Function

Anti-monomethyl Histone H2A.Z (Lys 7) is an antibody against monomethyl Histone H2A.Z (Lys 7) for use in WB, ICC, DB, IP.

Immunocytochemistry Analysis: A 1:2,000 dilution from a representative lot detected monomethyl Histone H2A.Z (Lys 7) in HeLa and NIH/3T3 cells.
Dot Blot Analysis: A 1:1,000 dilution from a representative lot detected monomethyl Histone H2A.Z (Lys 7) in Histone peptides with various modifications.
Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated monomethyl Histone H2A.Z (Lys 7) in 500 µg of HeLa acid extract.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

H2A.Z, also known as H2AFZ, is a variant of histone H2A that is highly conserved from yeast to humans. Like other histone variants, H2A.Z is encoded by a unique gene, resulting in a slightly divergent type of H2A with specialized functions. H2A.Z is involved in chromatin compaction by stabilizing the histone octamer within the nucleosome. It promotes gene expression through the creation of an open domain of chromatin that is more easily transcribed. H2A.Z is nonrandomly distributed throughout the genome, targeted primarily to pericentric heterochromatin, but excluded from the inactive X chromosome and the nucleolus. H2A.Z is associated with both euchromatin and facultative heterochromatin. DNA methylation can influence chromatin structure and effect gene silencing by excluding H2A.Z, and likewise, H2A.Z protects genes from DNA methylation. Thus, H2A.Z plays a role in regulating heterochromatin silencing and is involved in transcriptional control.

Immunogen

Epitope: Near N-terminal

KLH-conjugated linear peptide corresponding to human monomethyl Histone H2A.Z (Lys 7) near the N-terminal.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa acid extract.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected monomethyl Histone H2A.Z (Lys 7) in 10 µg of HeLa acid extract.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~16 kDa observed